Protecting the life of your herbicides in Southern Victoria

Author: Peter Boutsalis (School of Agriculture, Food & Wine, University of Adelaide, Plant Science Consulting P/L), Christopher Preston, Samuel Kleemann and Gurjeet Gill (School of Agriculture, Food & Wine, University of Adelaide) | Date: 25 Aug 2016

ɸExtra technical comment by Protech Consulting Pty Ltd

Herbicide resistant grass weeds in Southern Victoria

GRDC funded random weed surveys of cropping regions across southern Australia are being regularly conducted. The methodology involves collecting weed seeds from paddocks chosen randomly at pre-determined distances. Weed seeds are collected and tested in pot trials the following winter. Random surveys have shown that the greatest herbicide resistance is in annual ryegrass. The incidences of resistance in the south-western Victoria (SW Vic) region, as identified by the University of Adelaide are presented in Table 1. 

In ryegrass, low levels of resistance were detected to trifluralin and triallate. This is the first confirmation of triallate resistance in SW Vic. No resistance to Boxer Gold, Propyzamide or Sakura was detected. 

In contrast, substantial levels of resistance to cereal selective Group A and B herbicides was detected. Axial was more effective than Hoegrass. Additionally, resistance to clethodim was low. 

Ninety-six per cent of paddocks contained sulfonylurea resistant ryegrass, whereas 33 per cent of paddocks contained imidazolinone (IMI)-resistant ryegrass. This indicates that about 60 per cent of sulfonylurea resistant ryegrass populations could be controlled with Intervix. A herbicide resistance test would reveal if Intervix is effective.

In wild radish, almost half the samples collected exhibited resistance to Gleanɸ; it’s likely that cross-resistance to other Group B herbicides would occur. Of concern was that 7 per cent exhibited resistance to 2,4-D. No resistance to Glyphosate or Atrazine was detected. .

ɸGlean is no longer a registered product however other chlorsulfuron products are currently registered in Australia.

Map showing paddocks surveyed in Southern victoria.

Figure 1: Survey regions in Southern Victoria. Each point represents a paddock surveyed.

Table 1: The incidence of herbicide resistance in SW Vic as determined by a random weed survey of 120 paddocks in 2014 by the University of Adelaide. The data represents the percentage (%) of samples (one sample represents one paddock) where 20 per cent or greater survival was recorded in a pot trial. Each herbicide was applied at the field rate with the recommended adjuvants. 

 Pre-emergent herbicides
  Trifluralin Propyzamide Triallate Boxer Gold Sakura    
Ryegrass 2 0 10 0 0    
Post-emergent herbicides
Intervix Hoegrass Axial Select 250ml/ha Select 500ml/ha Glyphosate
Ryegrass 96 33 86 53 6 3 4
Atrazine 2,4-D Glyphosate      
Wild radish 43 0 7 0      

Getting the best out of pre-emergent herbicides

In the absence of effective post-emergent herbicides, ryegrass management has to rely on pre-emergent herbicides and non-chemical tactics. In getting the best out of pre-emergent herbicides it is important to understand some of their characteristics and how they will perform under different conditions.

Trifluralin and Stomp® (pendimethalin) have low water solubility so tend to stay where they are applied. Therefore, they need to be placed in close proximity to the weed seed. These herbicides are also volatile, so need incorporation shortly after they are applied to avoid losses. They bind tightly to organic matter including stubble. If there is too much stubble, some will need to be removed to get these herbicides to work effectively. 

Boxer Gold®(prosulfocarb + S-metalochlor) has high water solubility and will move readily through the soil. It typically requires 5 to 10mm of rainfall over a week to activate the herbicide. If heavy rainfall occurs after application, some crop damage may occur. Wheat is more sensitive than barley, so potential for damage will be greater in wheat crops. Boxer Gold® has medium binding to organic matter, so will move more readily in low organic matter soils. Boxer Gold®

has relatively short persistence, so late emerging weeds may be a problem in high rainfall zones.

Sakura® (pyroxasulfone) has lower water solubility, making it less likely to move in soil. It requires more rainfall than Boxer Gold® to activate; 10-15mm. Sakura® is not bound tightly to soil, but its low water solubilty means that it is normally not highly mobile. However, in soils with low organic matter or after high rainfall events some crop damage may occur. Sakura® is active for an extended period of time.

Avadex Xtra®(triallate) on its own will only control ryegrass at high rates. It is volatile and requires incorporation. Avadex Xtra® is more mobile in soil than trifluralin and binds less tightly to organic matter. Avadex Xtra® is primarily absorbed through the coleoptile rather than the roots, so it controls deeper emerging weeds.

Rustler® and Imtrade Edge 900WG® are the only propyzamide products registered in canola. Propyzamide is similar to Sakura®in its behaviour. It has low water solubility and medium binding to organic matter in the soil. This means it usually does not move far through the profile, but can do so with heavy rain. Canola is sown shallower than wheat, so the herbicide is generally placed within closer proximity to the seed. Therefore, Rustler damage to canola is more likely with high rainfall.

We conducted a series of six trials in high rainfall zones to determine how to get the most out of pre-emergent herbicides. Pre-emergent herbicides generally perform better in lower rainfall zones, because there is not the extended germination of ryegrass that occurs in high rainfall zones. Therefore, these trials demonstrate the full value of the products. The trials also examined season long control of annual ryegrass by counting the number of seed heads present at harvest. As a rule of thumb it is necessary to reduce ryegrass populations by 97 per cent or more in order to keep the seed bank at the same level.

The results are in Figure 2 as a box and whiskers plot where the mean is the line in the middle of the box and the whiskers are the range of the results. Some of these trials were conducted with populations that are resistant to trifluralin, affecting the performance of this herbicide.

Sakura® on average performed better in these high rainfall environments than did Boxer Gold® or trifluralin. Adding Avadex Xtra® to any of the other pre-emergent herbicides improved control. New registrations for Boxer Gold® will provide increased flexibility in gaining higher control of annual ryegrass populations.

Whisker box chart showing reduction in ryegrass spikes

Figure 2: Season-long control of annual ryegrass by pre-emergent herbicides in six trials conducted in high rainfall zones.

Key resistance management considerations

Cross-resistance patterns

Herbicide cross-resistance patterns differ between species. Ryegrass resistant to one FOP herbicide is almost always resistant to all other FOPs even if they have never been used. However, brome can be resistant to Targa® but not to Verdict®, or resistant to Select® but not to Verdict®. Barley grass also tends to follow the same pattern as brome. Furthermore in wild oats, there are cases where Targa® can control Topik® and Axial® resistant wild oats. Other cross-resistance patterns can give resistance to Topik, Verdict and Select but not to Axial.  In broadleaf weeds such as wild radish, Indian hedge mustard and sowthistle, diverse resistance patterns can occur between one or more herbicides such as Ally®, Gleanɸ, Eclipse®, Intervix®, etc. There are situations where exclusive use of one Group B herbicide (eg. Gleanɸ) has resulted in broad cross-resistance in a broadleaf weed species so that when another Group B is herbicide is used for the first time (even if in a different chemical class eg. Eclipse or Intervix), the weed is highly resistant. Cross-resistance patterns in the same species can also differ between neighbouring paddocks depending on the origin of the resistance. Herbicide resistance testing allows growers to identify suitable herbicides that are still highly effective on their weed populations.

ɸGlean is no longer a registered product however there are plenty of other chlorsulfuron products registered.

Rotating herbicides

If an herbicide is working well, it can be difficult to convince a grower to change to another. However, rotating herbicides aids in reducing the risk of resistance to any one herbicide. Heavy use of Group B herbicides in the upper Yorke Peninsula in SA led to the rapid development of resistance in Indian hedge mustard to all Group B chemistries. Subsequently rotating and relying heavily on Group I chemistry proved effective in the short-term but soon after Indian mustard with multiple resistance to Group B and I herbicides became prevalent. Herbicide modes of action must be rotated to keep chemistries working for as long as possible. Even if Group B’s are still effective, rotate with Group C, F, G, H, and I herbicides to reduce the risk of resistance to Group B herbicides.

Herbicide rate

Some herbicides have a rate range in the label. Using the highest rate within the rate range can often improve weed control. Weeds with weak resistance mechanisms can often be killed with higher rates. This is particularly common for clethodim and glyphosate resistance. 

Ensuring optimum coverage is also essential to maximise weed control. Using high rates with poor coverage can have the same effect as applying a lower rate. To maximise coverage and herbicide performance, it is important to aim to spray during ideal weather conditions with the correct nozzles, speed, water volume, water quality and adjuvant.

Herbicide resistance testing 

The only way to determine which herbicides will still control a weed population is to conduct a test. Some growers find it convenient to assume they have herbicide resistance to all Group A and B herbicides and use products such as Sakura® or Boxer Gold® because there is no confirmed field resistance to either product. However, this can be a costly exercise and there may be cheaper, older herbicides that still work but have been overlooked. 

Growth stage

Weeds are not always resistant at all growth stages. The type of resistance mechanism present and the growth stage of the plant influences herbicide efficacy. Numerous trials have shown that herbicide resistant weeds can sometimes be killed or heavily damaged if treated at the seedling stage. Clethodim has been shown to control some DIM resistant ryegrass biotypes at the 2-leaf stage but not at the tillering stage. Improved control of Group A resistant brome has been shown when 2-leaf seedlings are treated compared with poor control of tillering plants. Group I resistant wild radish can often be better controlled at the 2-3 leaf stage rather than at the 5-6 leaf stage. A common strategy by some growers is to delay application of post-emergent herbicides to maximise germination from the seedbank in order to ‘treat all the weeds’. This strategy is sound where there is no herbicide resistance present. However, reduced control of older plants that are herbicide resistant can occur. For weeds with staggered germination particularly brome, wild oats and wild radish, multiple herbicide timings (with different chemistries) should be used.

Non-herbicide techniques

Strategies that do not rely on herbicides alone can ‘take the pressure off’ herbicides and reduce the risk of resistance. In most cases, relying on herbicides alone will lead to resistance. Extensive research is occurring in the area of non-herbicide weed control techniques which are often termed integrated weed management (IWM) strategies. Maximising crop competition with weeds is an important factor. Weeds often occupy gaps in crops. Cereals are usually more competitive than broadleaf crops. Barley tends to be the most competitive cereal, whereas durum is the least competitive. Substantial differences between varieties of the same crop species can also occur so growing the best suited variety for your region is important. Seed with poor crop vigour will not compete effectively with weeds. A germination test will not test for vigour; vigour testing is important. Other factors which can optimise crop seed germination, emergence and development include correct sowing time, correct seeding depth, fertiliser timing and placement, and pest and disease management. 

If a crop is infested with weeds, urgent action is needed because replenishing the seed bank can be disastrous. Tactics to sacrifice part of the crop to prevent the replenishment of the seedbank can include cutting for hay, silage or green manuring. Harvesting the crop and removing or killing weed seed by the use of chaff carts, Harrington Seed Destructor (HSD) or placing the chaff in windrows for burning will also reduce the weed burden. 

A closer look at glyphosate resistance

Nationally, the number of species and individual cases of confirmed glyphosate resistance continues to increase with the greatest number of cases in ryegrass. Most cases of glyphosate resistant ryegrass have been detected in winter grain crops (300 cases), fencelines and roadsides (100 cases each). Cases of resistance in new species such as sowthistle and wild radish are of particular concern. 

Line graph showing reduced efficacy of glyphosate

Reduced efficacy of glyphosate

There are numerous reasons for the poor performance of glyphosate, a common one being herbicide resistance. Resistance to glyphosate can range from weak resistance to strong resistance. Plants with weak resistance may be controlled with higher label rates, but this strategy should not be overused because weeds can develop resistance to very high rates. One Victorian roadside population has survived 20L/ha glyphosate in pot trials. 

Table 2: Percent survival (%) of a selection of grower resistance tests from 2013 and 2014 treated with glyphosate (540g ai/L). Data ranked according to per cent survival at 1000ml/ha. 

Town State 1000 1500 2000
Wagin WA 5 0 0
Yendon NSW 5 5 0
Griffith NSW 5 5 5
Dowerin WA  5
Lake Grace WA 10 
Yendon NSW 20  20 
Yendon NSW 20 0 0
Deniliquin NSW 20 5
Temora NSW 20
Goomalling WA 20
Goomalling WA 20 20 
Calingirri WA 20 0 0
Griffith NSW 20 5 5
Griffith NSW 25 0 0
Yendon NSW 40 20 0
Badgingarra WA 50 5 0
Griffith NSW 55 5 0
Ballidu WA 80 70 70
Griffith NSW 80 60 0
Nhill VIC 80 80 5
Naracoorte SA 90 55 0
Calingirri WA 100 90 90
Data courtesy of Peter Boutsalis, Plant Science Consulting

Glyphosate is usually absorbed within 24 hours of application and moves readily in the phloem of actively growing plants. Application of glyphosate in the morning can result in greater uptake than application at night. Greater glyphosate activity is usually observed in actively growing young plants. On larger plants, higher rates are required to maintain good efficacy. Herbicide uptake can be restricted when plants are stressed. Factors that can cause stress include frost, moisture (drought or waterlogging), temperature, nutrition and pest damage. Pot trials have shown that glyphosate activity is often reduced as ambient temperature increases. In ryegrass it has been observed that the optimum daily temperature for glyphosate activity ranges between the low teens and mid-twenties. These findings have been observed in unstressed, two to three leaved ryegrass growing in pots. 

Factors that limit the contact of glyphosate with a target weed include poor coverage (water rates, nozzle selection, application on very dense populations or wet leaves), poor water quality or application onto dust covered plants. Glyphosate is readily bound to soil particles present as dust or in dirty water. 

Applying glyphosate on herbicide resistant weeds that are stressed or exposed to sub-optimum herbicide concentrations (reduced coverage, dust and so on) can result in poor control. A combination of factors that reduce glyphosate efficacy on plants with weak resistance mechanisms can elevate the resistance levels. Testing for glyphosate resistance via seed or plants (Quick-Test) can aid in making future weed control decisions. A test can highlight the presence of weak glyphosate resistance and therefore indicates whether higher rates could be effective. The identification of strong resistance can aid in convincing growers to adopt alternative strategies to combat the problem. 
Line graph with whisker mean showing percentage of survival
Figure 4: Glyphosate resistance mechanisms are additive. Dose response of ryegrass populations with a target site mutation, SLR 77, (●), the translocation resistance mechanism, NLR 70, (■), and the F1 cross between SLR 77 and NLR 70 (♦) compared with the susceptible population VLR1 (○).  Increased resistance occurs when two mechanisms of glyphosate resistance occur in the same plant. 

Improving glyphosate efficacy

Using higher label rates can often improve weed control. Weeds with weak glyphosate resistance mechanisms can often be killed with higher label rates. Additionally, higher rates can help counteract poor application, improve control of older plants, stressed plants or overcome reduced efficacy caused by using poor quality water or by treating plants covered by dust. Higher label rates can also improve glyphosate activity of plants exposed to higher temperatures that can arise in early autumn or late spring.

Our findings have shown that although glyphosate resistant weeds are resistant at all growth stages, seedlings are more sensitive than multi-tillered plants. Numerous trials have shown that herbicide resistant weeds are often killed or heavily damaged if treated at the seedling stage. A common strategy by some growers is to delay application of glyphosate to maximise germination from the seedbank in order to 'treat all the weeds'. This strategy can be effective if the weeds are not herbicide resistant or stressed. However, reduced control of older plants that are herbicide resistant can occur if rates are not sufficiently high or weeds are stressed. In weed species that exhibit staggered germination such as brome, wild oats and wild radish, multiple herbicide timings are recommended. The type of resistance mechanism(s) present and more importantly the level of resistance it confers can also influence glyphosate efficacy.

Contact details

Peter Boutsalis 
University of Adelaide, Waite Campus, Glen Osmond SA 5064

Herbicide resistance testing: 
Peter Boutsalis, Plant Science Consulting 
0400 664 460