Managing insecticide resistance (Helicoverpa armigera, green peach aphid, redlegged earth mite) and an update on Russian wheat aphid

Author: Paul Umina (cesar; University of Melbourne), Siobhan de Little (University of Melbourne), Lisa Kirkland (University of Melbourne), Elia Pirtle (University of Melbourne), Matthew Binns (cesar) and James Maino (University of Melbourne) | Date: 27 Feb 2018

Take home messages

Insecticide resistance issues continue to outpace availability of novel control options.

Aphids:

  • Green peach aphid (GPA) has acquired resistance to neonicotinoids.
  • Pirimicarb is now mostly ineffective against GPA due to resistance, but remains effective against other crop aphids, highlighting the importance of correct species identification.
  • A variety of insecticide seed treatments have been shown to control Russian wheat aphid, with the length of protection differing between products. No seed treatments are registered, however use of products containing 600 g/L imidacloprid as their only active constituent are allowed under permit PER82304.

Helicoverpa armigera:

  • Insecticide control of H. armigera is complicated due to field resistances and increased selection pressure to important insecticide products.
  • The implementation of a recently published Resistance Management Strategy is vital to maximising the long-term viability of chemical options.

Redlegged earth mite (RLEM):

  • Insecticide resistance in RLEM has been detected for first time in eastern Australia.
  • Synthetic pyrethroids (SPs) are completely ineffective against SP-resistant RLEM populations, while some efficacy remains for organophosphates (OPs) against OP-resistant RLEM populations.

Background

Insecticide resistance issues in broadacre cropping continue to outpace the availability of novel control options. In this paper, we discuss the latest findings on two major pest species that have developed resistance to key chemical groups, the green peach aphid (Myzus persicae, GPA) and the redlegged earth mite (Halotydeus destructor, RLEM), and present a new Resistance Management Strategy developed specifically for Helicoverpa armigera in grains. We also provide new research on the efficacy of seed treatments against Russian wheat aphid (Diuraphis noxia, RWA).

Green peach aphid acquires new resistances

Green peach aphid is a widespread and damaging pest of canola and a range of pulse crops, causing damage by feeding and transmitting viruses. Five chemical subgroups are registered to control GPA in grain crops: carbamates (group 1A); synthetic pyrethroids (SPs - group 3A); organophosphates (OPs - group 1B); neonicotinoids (group 4A); and sulfoxaflor (group 4C). Paraffinic spray oils are also registered for suppression of GPA.

Together with CSIRO, cesar have been mapping the extent of insecticide resistance in GPA across Australia for the past few years with strategic investment from GRDC. This ongoing resistance surveillance has continued to show high levels of resistance to carbamates and SPs that are widespread across Australia. Moderate levels of resistance to OPs have been observed in many populations, and there is evidence that resistance to neonicotinoids is spreading.

Despite widespread resistance to the carbamate, pirimicarb, in GPA populations (Figure 1), this insecticide remains important to the control of other canola aphids of similar appearance (e.g. cabbage aphid). Thus, it is important to properly identify aphids before spray decisions are made. Figure 2 highlights some key features that can be used to distinguish GPA from other similar species found on canola (with a hand lens). If a hand lens is unavailable, GPA will usually be found on lowest, oldest leaves, typically in sparse family groups, while turnip aphid & cabbage aphid are more commonly found in large colonies on flowering spikes.

Neonicotinoid resistance conferred by enhanced expression of the P450 CYP6CY3 gene was discovered in Australian GPA populations in 2016 by cesar and CSIRO researchers. Laboratory bioassays revealed these aphids to be ~10 times more resistant to a topical application of a neonicotinoid compared to a susceptible population. However, overseas GPA are known to carry an R81T gene mutation of the nicotinic acetylcholine receptor that confers ~1000 times resistance to neonicotinoids resulting in field control failures, as well as cross-resistance with group 4C chemicals such as sulfoxaflor. Australian GPA may acquire this high-level resistance if neonicotinoid selection pressures remain high, or if there is an incursion of overseas GPA carrying the R81T mutation.

Figure 1 (left) is a line graph showing the sensitivity of a typical Australian susceptible and resistant green peach aphid population to the synthetic pyrethroid, alpha-cypermethrinFigure 1 (middle) is a line graph showing the sensitivity of a typical Australian susceptible and resistant green peach aphid population to the carbamate, pirimicarbFigure 1 (right) is a line graph showing the sensitivity of a typical Australian susceptible and resistant green peach aphid population to the organophosphate, dimethoate

Figure 1. Sensitivity of a typical Australian susceptible and resistant green peach aphid population to the synthetic pyrethroid, alpha-cypermethrin (left panel), the carbamate, pirimicarb (middle panel) and the organophosphate, dimethoate (right panel).  RF = Resistance Factor

Figure 2 shows a picture of three aphids and the diagnostic differences between aphid species

Figure 2. To assess the applicability of pirimicarb to other non-resistance aphid species of similar appearance, green peach aphid should be distinguished using diagnostic traits.

New resistance management strategy for Helicoverpa armigera

Helicoverpa armigera is a major pest of grains crops. Direct feeding by H. armigera reduces yield of pulses, oilseeds, coarse grains and, occasionally, winter cereals. Losses come from direct weight loss through seeds being wholly or partly eaten. Grain quality may also be downgraded through unacceptable levels of chewed grain. Although widely distributed and recorded in all states and territories within Australia, H. armigera is more common in the northern or coastal regions of eastern Australia, particularly in warmer regions. In cooler regions, they are generally only problematic in summer.

There are over 200 insecticide products registered in Australia against H. armigera for grains, cotton and vegetable crops. The majority are from 3 chemical sub-groups with broad-spectrum activity:

  • carbamates (group 1A)
  • organophosphates (group 1B) and
  • synthetic pyrethroids (group 3A).

However, insecticides from group 6 (emamectin benzoate), group 22A (indoxacarb) and group 28 (chlorantraniliprole) are become more widely used in pulses due to high efficacy and low impact on natural enemies. Control is complicated because field populations are resistant to numerous insecticide groups (Table 1). Due to these factors, timing of chemical applications and coverage are critical, and growers need to understand how to minimise yield loss without furthering resistance levels.

Table 1. Products with label claims for Helicoverpa armigera (and Helicoverpa spp. generally) in Australian grain crops and current resistance status

IRAC MoA Group

Insecticide category

Active ingredient(s)

Example trade name(s)

Resistance in Australia

1A

Carbamates

methomyl, thiodicarb

Lannate®, Marlin®, Larvin®

Moderate – high (30-50%)

1B

Organophosphates*

chlorpyrifos

Chlorpos, LorsbanTM, Chlorpyrifos

Low – moderate (1-10%)

3A

Pyrethroids

alpha-cypermethrin, beta-cypermethrin, cypermethrin, deltamethrin, gamma-cyhalothrin, lambda-cyhalothrin, esfenvalerate, permethrin, bifenthrin

Alpha-Scud®, Astound®, Trojan®, Talstar®, Sumi-Alpha® Flex

Metabolic resistance is high (50-100%)

Target site resistance is low (<5%)

5

Spinosyns

spinetoram

SuccessTM Neo

Very low (<2%)

6

Avermectins

emamectin benzoate

Affirm®

11C

Bacillus thuringiensis

B.t. subsp. Kurstaki, B.t. subsp. aizawai

DiPel®, Delfin®, Costar®, Bacchus®

Low (<5%)

22A

Oxadiazines

indoxacarb

Steward®

Low, but increasing

(5-12%)

28

Diamides

chlorantraniliprole

Altacor®

Very low

(<1%)

No Group

Nuclear polyhedrosis virus

nuclear polyhedrosis virus

Gemstar®, Vivus® Max/Gold

No Group

Paraffinic spray oils

paraffinic oil

Canopy®

* Not registered to control H. armigera in grain crops.

Table adapted from: Science behind the Resistance Management Strategy for Helicoverpa armigera in Australian grains (NIRM, 2018). Data provided by NSW Department of Primary Industries with support from the Cotton Research and Development Corporation (CRDC) and the Grains Research and Development Corporation (GRDC).

A new Resistance Management Strategy (RMS) has recently been produced for H. armigera in Australian grain crops and will be available for the 2018 field season. This RMS was developed by the National Insecticide Resistance Management (NIRM) working group of the Grains Pest Advisory Committee (GPAC), and is endorsed by CropLife Australia.

The general rationale for the design of the strategy is that chickpeas and mung beans are currently, and for the foreseeable future, the crops in which the use of insecticides is most likely to have the greatest impact on the management of resistance in H. armigera populations. Therefore, the strategy is primarily focused on insecticide modes of action (MoA) rotation in these systems and is built around product windows for Altacor and Steward because:

  1. Altacor is at risk from dangerously high levels of over-reliance in pulses, but resistance frequencies are currently low.
  2. Steward is at risk due to genetic predisposition (high level genetic dominance and a metabolic mechanism). Pre-existing levels of resistance in NSW and QLD are present (with elevated levels in CQ during 2016-17). In addition, Steward is now off-patent in Australia which provides the opportunity for lower priced products to enter the market, which may further increase frequency of applications.

There are two RMS regions:

  1. Northern grains region (Belyando, Callide, Central Highlands & Dawson); and
  2. Central grains region (Balonne, Bourke, Burnett, Darling Downs, Gwydir, Lachlan, Macintyre, Macquarie & Namoi).
  • The RMS provides window-based recommendations common to Southern QLD, Central & Northern NSW because H. armigera moths are highly mobile and have the capacity to move between these regions, potentially increasing the risk of further exposing cohorts of insects previously selected for resistance.
  • We have limited knowledge of the likely risk of H. armigera occurrence in winter crops in the southern and western grains regions (Victoria, South Australia and Western Australia) because there has been little formal monitoring for this species in these regions. However, there is some historical data, and anecdotal records of H. armigera outbreaks in the southern region, which suggests that in some years and regions there is a risk of control failure and/or selection of resistance in the Helicoverpa population because of the presence of H. armigera.
  • No RMS is currently proposed for the southern and western grains regions. Biological indicators are that the risk of H. armigera occurring in winter crops, at densities where control failures may occur, is presently considered low. However, if required, the Central Grains region RMS may be adapted for H. armigera management in summer crops in these regions.

The new RMS for grain crops is not intended to ‘sync’ with the cotton IRMS. Recommended windows for use in the two industries do not align, and the level of insecticide used for Helicoverpa control in cotton is relatively small in comparison with the areas of winter and summer pulses potentially treated each year. It is considered that insecticide use patterns in cotton pose little risk to the ongoing management of resistance, relative to the risk posed by year-round, high level use in grains.

For further information on the cotton IRMS go to the 2017-18 Cotton Pest Management Guide, CottonInfo

Resistance in redlegged earth mites spreads to eastern Australia

The redlegged earth mite (Halotydeus destructor, RLEM) is an important pest of germinating crops and pastures across southern Australia. Four chemical sub-groups are registered to control RLEM in grain crops: organophosphates (OPs) (group 1B); synthetic pyrethroids (SPs) (group 3A); phenylpyrazoles (group 2B); and neonicotinoids (group 4A). The latter two are registered only for use as seed treatments (Umina et al., 2016).

After remaining confined to WA for a decade, insecticide resistance in RLEM was detected for the first time in eastern Australia in 2016 (Maino, Binns and Umina, 2017). In WA, resistance to SPs is widespread, while OP resistance is comparatively more restricted (Figure 3). In 2016, following reports of a field control failure in the upper south-east district in South Australia; resistance testing determined this South Australian population was resistant to SPs and OPs (Figure 4). In 2017, two additional SP resistant populations were confirmed on the Fleurieu peninsula (~30 km apart from each other, and ~200km from the 2016 detection).

Figure 3 is a map of Australia showing the current known distribution of redlegged earth mite in Australia (adapted from Hill et al. 2012) shown as filled circles, overlaid with the known distribution of synthetic pyrethroid (SP) and organophosphate (OP) resistance across Australia at 2017. Shows the majority in WA with one or two sites in SA

Figure 3. The current known distribution of redlegged earth mite in Australia (adapted from Hill et al. 2012) shown as filled circles, overlaid with the known distribution of synthetic pyrethroid (SP) and organophosphate (OP) resistance across Australia at 2017.

All SP resistant populations tested to date have been found to possess a target site mutation on the para-sodium channel (Edwards et al., 2017). This mutation confers high level SP resistance (~200,000 times the resistance of a susceptible population) leading to complete spray failures (Figure 4). In contrast, the mechanism conferring OP resistance has not yet been resolved, but resistance is comparatively less than SP resistance, such that OP efficacy will be reduced but not lost entirely.

Figure 4 is two line graphs showing the concentration-mortality curves for redlegged earth mite from susceptible (DC01) and resistant (SA01) populations when exposed to a synthetic pyrethroid - bifenthrin (A) - and an organophosphate - omethoate (B) - after 8 h exposure. Vertical bars denote standard errors. Lines represent fitted values from fitted logistic regression models.

Figure 4. Concentration-mortality curves for redlegged earth mite from susceptible (DC01) and resistant (SA01) populations when exposed to a synthetic pyrethroid - bifenthrin (A) - and an organophosphate - omethoate (B) - after 8 h exposure. Vertical bars denote standard errors. Lines represent fitted values from fitted logistic regression models.

Testing control methods for Russian wheat aphid

Russian wheat aphid (Diuraphis noxia, RWA) was first detected in Australia in 2016. The host range of RWA includes more than 140 species of cultivated and wild plants within the family Gramineae (grasses). These include wheat, barley, triticale, rye, oats, pasture grasses and wild genera including Poa, Bromus, Hordeum, Lolium, Phalaris and others. Wheat and barley are most susceptible, while triticale, rye and oats are less susceptible.

Unlike other cereal aphids that damage plants by removing nutrients, RWA also injects salivary toxins during feeding that cause rapid, systemic phytotoxic effects on plants, resulting in acute plant symptoms and potentially significant yield losses. Even a few aphids can cause plant damage symptoms to appear as early as 7 days after infestation. These include:

  • white and purple longitudinal streaks on leaves;
  • curled, rolled or hollow tube leaves;
  • stunted growth or flattened appearance;
  • discolored leaves;
  • hooked-shaped head growth from awns trapped in curling flag leaf; and
  • bleached heads.

Insecticide seed dressingsɸ can be effective to combat RWA infestations in establishing cereal crops. cesar have tested the relative efficacy and length of activity of various insecticide seed dressings in wheat against RWA, and compared this with another important cereal aphid pest, the oat aphid (Rhopalosiphum padi).

Seed dressings tested provided effective aphid control up to 5 weeks after emergence, with higher rates generally providing several weeks extra protection over lower rates of the same product. Oat aphids generally persisted and reproduced on wheat at an earlier time-point than RWA, suggesting that RWA is less tolerant to the insecticide seed dressings tested. This suggests that management of cereal aphids in Australia using insecticide seed dressings is likely to achieve similar, if not better, control of RWA as oat aphid.

ɸNo insecticides(seed dressings or in-crop application) are currently registered for use in Australia, but use is permitted under the following permits: PER81133, PER82304 and PER83140.

Useful resources

Resistance Management Strategy for the Green Peach Aphid in Australia Grains, GRDC

Tips & Tactics: Russian Wheat Aphid, GRDC

Redlegged earth mite RMS – Western Australia, GRDC

Redlegged earth mite RMS – NSW, SA, Tasmania and Victoria, GRDC

References

Edwards, O. R. et al. (2017) ‘A genomic approach to identify and monitor a novel pyrethroid resistance mutation in the redlegged earth mite, Halotydeus destructor’, Pesticide Biochemistry and Physiology, p. doi: https://doi.org/10.1016/j.pestbp.2017.12.002.

Hill, M. P. et al. (2012) ‘Understanding niche shifts: Using current and historical data to model the invasive redlegged earth mite, Halotydeus destructor’, Diversity and Distributions, 18(2), pp. 191–203. doi: 10.1111/j.1472-4642.2011.00844.x.

Maino, J.L., Binns, M. and Umina, P. A. (2017) ‘No longer a west-side story – pesticide resistance discovered in the eastern range of a major Australia crop pest, Halotydeus destructor (Acari: Penthaleidae)’, Crop and Pasture Science.

Umina, P. A. et al. (2016) ‘Science behind the resistance management strategy for the redlegged earth mite in Australian grains and pasture’.

Acknowledgements

The research presented here is made possible by the significant contributions of growers through both trail cooperation and the support of the Grains Research and Development Corporation. The authors would like to thank them for their continued support. A special thanks to members of the NIRM working group, especially Lisa Bird (NSW DPI) and Melina Miles (QDAF) for their contributions and reviews in relation to H. armigera. We would also like to acknowledge other collaborators including Julia Severi (cesar), Ary Hoffmann (University of Melbourne), Owain Edwards (CSIRO), Jenny Reidy-Crofts (CSIRO), Svetlana Micic (DAFWA), and Alan Lord (DAFWA). The authors also acknowledge the assistance of Ken McKee & David Landmeter (Syngenta Australia), Shane Trainer (Bayer Crop Science) and Colin Edmondson (Advanta Seeds).

Contact details

Dr Paul Umina
cesar Pty Ltd, 293 Royal Parade, Parkville VIC
Phone: 03 9349 4723
Email: pumina@cesaraustralia.com

Dr James Maino
cesar Pty Ltd, 293 Royal Parade, Parkville VIC
Phone: 03 9349 4723
Email: jmaino@cesaraustralia.com

Twitter handle: @cesaraustralia

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